Characterization by gas chromatography/mass spectrometry of sterols in saccharomyces cerevisiae during autolysis.

Yeast autolysis affects membrane stability and induces a release of vacuolar enzymes into the cell cytoplasm. Consecutively, it was important to study the evolution of sterol content in Saccharomycescerevisiae for a fourteen day period of accelerated autolysis. Unesterified and esterified sterols were analyzed both in the biomass and in the autolysis medium. Ten sterols were identified by gas chromatography/mass spectrometry. A second group of six sterols was separated and partially characterized. Among the first group of 10 sterols, a dehydroergosterol was identified as ergosta-5, 7,9(11),22-tetraen-3beta-ol, not yet charaterized in S. cerevisiae. Yeast autolysis induced a decrease of esterified sterol content, especially first intermediates in the sequence of the ergosterol biosynthesis, as zymosterol. In contrast, the yeast autolysis resulted in the release of a low quantity of sterols into the medium. At the end of the fourteenth day of autolysis, 0.015% of the total sterol content of the initial biomass was found in the medium.     

松毛虫赤眼蜂对柞蚕灰卵的寄生选择与适应性

为明确柞蚕Antheraea pernyi灰卵(工厂化繁育赤眼蜂过程中广泛存在的一种感病寄主卵)对松毛虫赤眼蜂Trichogramma dendrolimi寄生选择与适应性及其繁育子代蜂寄生能力的影响,以柞蚕灰卵作为供试寄主,健康卵作为对照,在无选择和双向选择条件下研究松毛虫赤眼蜂对其的寄生选择与适应性,并比较灰卵和健康卵繁育的子代蜂对0、1、2、3日龄米蛾Corcyra cephalonica卵的寄生能力。结果表明,在无选择条件下,松毛虫赤眼蜂在灰卵上的寄生率、羽化率、单卵出蜂数和总蜂数分别为30.0%、54.1%、39.3头和48.7头,而在健康卵上分别达到96.0%、93.0%、82.5头和96.8头。在双向选择条件下,松毛虫赤眼蜂在灰卵上的寄生率、羽化率、单卵出蜂数和总蜂数分别为33.3%、27.1%、24.7头和52.2头,而在健康卵上分别为68.0%、86.3%、60.6头和74.2头。在无选择和双向选择条件下,松毛虫赤眼蜂在灰卵和健康卵上的发育历期和后代雌性比均不存在显著差异,但灰卵繁育的子代蜂对1、2日龄米蛾卵的寄生数则显著低于健康卵繁育的子代蜂,且后者对米蛾卵的寄生数随着米蛾卵日龄的增加呈逐渐下降趋势,而灰卵繁育的子代蜂对0、3日龄米蛾卵的寄生数要显著高于1、2日龄的。表明柞蚕灰卵会对松毛虫赤眼蜂的寄主选择与适应性以及子代蜂的寄生能力产生不良影响。     

大力推广方格蔟,提高蚕茧质量

本文在总结湖州雠主格蔟成绩的基础上，针对当前在方格蔟推广上存在的问题，提出了大力推广方格蔟，提高茧质的建议。     

魔芋软腐病病原菌TaqMan荧光探针PCR技术的建立及应用

为实现对田间土壤软腐病病原菌的定量检测,基于魔芋软腐病优势病原菌胡萝卜软腐果胶杆菌胡萝卜亚种Pectobacterium carotovorum subsp. carotovorum的FyuA基因序列,设计特异性引物PCC1/PCC2/PCC3,建立TaqMan荧光探针实时荧光定量PCR技术,并对魔芋根系土壤中软腐病病原菌进行动态监测。结果显示:基于FyuA基因序列设计的引物特异性好,仅能特异性检出胡萝卜软腐果胶杆菌胡萝卜亚种;当模拟带菌土壤中病原菌浓度低至1.88 CFU/g时也能检出,灵敏度高;发病魔芋根际土壤中软腐病病原菌检出率为100.00%,病原菌DNA浓度最高达到了7.52×10~7ng/μL,健康魔芋根际土壤中也存在病原菌,检出率为40.00%;不同种植模式中,林下魔芋土壤中软腐病病原菌数量较少;连作时间与病原菌数量、病情指数存在正相关关系,连作时间越长,病原菌积累越多,魔芋病情指数也越高,魔芋连作4年土壤中病原菌DNA浓度最高达到4.03×10~4ng/μL;对魔芋土壤软腐病病原菌进行全年监测,病原菌数量随着月份增长逐渐上升,在8—10月达到峰值543.20 ng/μL后下降,病原菌数量与魔芋病情指数变化规律一致,但田间魔芋软腐病的发生相对滞后。表明建立的TaqMan荧光探针实时荧光定量PCR技术可用于田间魔芋软腐病的监测。     

新疆棉花超高产光合生理基础研究

1996～ 1999年对新疆棉花超高产 (皮棉 2 2 50kg·hm-2 以上 )光合性能研究结果表明 ,盛铃期群体光合速率 (CAP)生育期变化为单峰型曲线 ,盛花结铃期达最大值 4 .0 5gCO2 ·m-2 h-1 ,平均值为 2 .63gCO2 ·m-2 h-1 ,盛花、结铃、吐絮期的 CAP 与生物学产量、皮棉产量呈显著正相关。盛铃期叶面积指数最大值为 3 .7～ 3 .9。一天内棉叶净光合时间长达 13 .5h,早晚比普遍棉田光合延长 2 0min左右。未发现光合午休和光饱和现象。高产棉田夜间 (2 1:2 0 )呼吸速率最大值为 4 .33mgCO2 ·dm-2 h-1 ,平均值为 3 .0 0mgCO2 ·dm-2 h-1 ,分别比普通棉田低2 2 %和 17.6 % ,扣除呼吸消耗 ,净光合比普通棉田高 58.4 %。其呼吸消耗占白天总光合的 8.4 %。低于普通棉田 15.5 % ,也低于内地棉区 12 %～ 2 0 %。新疆高产棉田群体的库容量增加 ,光合产物的贮备能力强 ,生物学产量高达 1.14× 10 4～ 1.44× 10 4kg·hm-2 ,有效铃 (5g以上 )高达 12 0×10 4个·hm-2 ,经济系数 41.52 %～ 44 .1% ;单位叶面积的果节数、有效铃、生殖器官干重分别为10 4～ 137个·m-2 ,32～ 49个·m-2 ,0 .2 30 3～0 .3361kg·m-2 。源强库大 ,源库协调是新疆棉花高产的生理基础。     

水杉人工林抚育间伐的效果比较

在１５ａ生水杉人工林内设立标准地，定量机械间伐，定期测定生长量，对抚育间伐７ａ后的效果及经济效益进行分析，结果表明：（１）不同初植密度的林分采用较小的强度ｐｎ＝２５％，３３％进行间伐，与对照林分相比，平均每公顷生长量分别提高５．９７，９．２７ｍ３，增加９．５７％，１４．８７％；采用较大强度ｐｎ＝５０％进行间伐，与对照林分相比，平均每公顷生长量下降９．７ｍ３，减少１５．５７％。（２）抚育间伐过的林分（间伐强度ｐｎ＝２５％，３３％，５０％）与对照林分相比，单位面积保留木平均生长率分别提高１．３４％，２．００％，１．９７％。（３）抚育间伐的林分与对照林分相比，平均每公顷总产值和利润分别增加６２７８，５７８４元，增长７．７％，９．５％。     

Potassium fertilization improves apple fruit (Malus domestica Borkh. Cv. Fuji) development by regulating trehalose metabolism

Potassium (K) fertilization and antioxidant enzymes both positively influence plant growth and development. However, it is not known whether K treatment improves fruit development via increasing soluble sugar. In this study, K-treated apple (Malus domestica Borkh.) fruit was harvested from 60 to 150 days after full bloom and was analyzed for ROS production and trehalose metabolism. The results show that K fertilization improved fruit firmness, increased growth according to several parameters, increased soluble sugar content, and decreased ROS production. The ascorbate metabolic pathway more effectively reduced ROS production than catalase and peroxidase (POD) did under K treatment. Trehalose-treated fruit also showed higher activity of ascorbate-related enzymes (DHAR, GR, and APX) compared with non-treated fruit. The changes of antioxidant enzyme activity in trehalose-treated fruit corresponded to those in K-treated fruit. Moreover, trehalase (TREH) activity in fruit was notably reduced by K treatment. This demonstrates that K influences ROS production via regulating trehalose content and TREH activity in fruit. This study provides new insight into the K mechanism which improves fruit development, including fruit firmness and size.     

Different genes can be responsible for crown rot resistance at different developmental stages of wheat and barley

Crown rot, caused by several Fusarium species, is one of the most damaging diseases in wheat and barley. Growing resistant varieties has long been recognised as an integral part in effectively managing the disease. One of the factors hindering the progress of breeding for crown rot resistance is the lack of a reliable and high throughput bioassay that allows rapid and accurate assessment of large numbers of genotypes so that highly susceptible materials can be quickly rejected and potentially resistant lines identified for more focused further assessments. We developed a method which, by growing several inoculated seedlings wrapped in a single piece of moist paper towel, offers significant advantages over all of the existing methods. The new soil-less assay takes only about two weeks, requires very little space, and removes variability associated with the use of soil/potting mixes. Results from the new assay are highly reproducible and agree well with known field performances of different varieties. However, mapping studies conducted using the new soil-less assay did not detect all of the quantitative trait loci found with a soil-based assay. These results show that, although different resistance genes may all contribute to the performance of a variety, caution should be used when comparing results from different assays.     

浅谈湖南湿地生态功能及作用

指出了湿地作为一种重要的自然资源,拥有丰富的生物多样性,具有巨大的生态功能和效益。总结了湖南省湿地污染物净化与过滤、调节气候、蓄水、调节径流、均化洪水、提供动植物栖息地以及维持区域生态平衡等生态功能,分析了湿地对湖南建设的作用。